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GenScript corporation anti-flag g1 affinity resin l00432
Anti Flag G1 Affinity Resin L00432, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-flag+g1+affinity+resin+l00432/anti+flag+g1+affinity+resin/pm38503280-766-24-29
Average 90 stars, based on 1 article reviews
anti-flag g1 affinity resin l00432 - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Centrifugation:

Article Title: A gut-derived hormone regulates cholesterol metabolism.
Article Snippet: Article

Article Title: MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria-dependent dephosphorylation of EGFR.
Article Snippet: Cells were lysed in ice-cold lysis buffer (50 mmol/L TrisHCl [pH 7.5], 150mmol/LNaCl, 1% Triton X-100, 1mmol/L EDTA) with 1 × protease inhibitor cocktail (C0001, TargetMol, Boston, MA, USA) for 30 min. Heterologously expressed MFN2-Flag and EGFR-HA were subjected to anti-Flag G1 affinity resin (L00432, GenScript, Nanjing, Jiangsu, China) or anti-HA Magnetic Beads (#88836, Thermo-Fisher Scientific, Waltham, MA, USA).

Article Title: MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria‐dependent dephosphorylation of EGFR
Article Snippet: Cells were lysed in ice‐cold lysis buffer (50 mmol/L Tris‐HCl [pH 7.5], 150 mmol/L NaCl, 1% Triton X‐100, 1 mmol/L EDTA) with 1 × protease inhibitor cocktail (C0001, TargetMol, Boston, MA, USA) for 30 min. Heterologously expressed MFN2‐Flag and EGFR‐HA were subjected to anti‐Flag G1 affinity resin (L00432, GenScript, Nanjing, Jiangsu, China) or anti‐HA Magnetic Beads (#88836, Thermo‐Fisher Scientific, Waltham, MA, USA).

Article Title: The Large Nonstructural Protein (NS1) of Human Bocavirus 1 Directly Interacts with Ku70, Which Plays an Important Role in Virus Replication in Human Airway Epithelia
Article Snippet: The supernatant was then collected, of which 80 μL was boiled in loading buffer as an input control, and the remaining supernatant was incubated with 100 μl of prewashed anti-Flag G1 affinity resin (number L00432; GenScript, Piscataway, NJ) followed by rotation at 4°C overnight.

Protein Binding:

Article Title: A gut-derived hormone regulates cholesterol metabolism.
Article Snippet: Article

Article Title: MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria-dependent dephosphorylation of EGFR.
Article Snippet: Cells were lysed in ice-cold lysis buffer (50 mmol/L TrisHCl [pH 7.5], 150mmol/LNaCl, 1% Triton X-100, 1mmol/L EDTA) with 1 × protease inhibitor cocktail (C0001, TargetMol, Boston, MA, USA) for 30 min. Heterologously expressed MFN2-Flag and EGFR-HA were subjected to anti-Flag G1 affinity resin (L00432, GenScript, Nanjing, Jiangsu, China) or anti-HA Magnetic Beads (#88836, Thermo-Fisher Scientific, Waltham, MA, USA).

Article Title: MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria‐dependent dephosphorylation of EGFR
Article Snippet: Cells were lysed in ice‐cold lysis buffer (50 mmol/L Tris‐HCl [pH 7.5], 150 mmol/L NaCl, 1% Triton X‐100, 1 mmol/L EDTA) with 1 × protease inhibitor cocktail (C0001, TargetMol, Boston, MA, USA) for 30 min. Heterologously expressed MFN2‐Flag and EGFR‐HA were subjected to anti‐Flag G1 affinity resin (L00432, GenScript, Nanjing, Jiangsu, China) or anti‐HA Magnetic Beads (#88836, Thermo‐Fisher Scientific, Waltham, MA, USA).

Article Title: The Large Nonstructural Protein (NS1) of Human Bocavirus 1 Directly Interacts with Ku70, Which Plays an Important Role in Virus Replication in Human Airway Epithelia
Article Snippet: The supernatant was then collected, of which 80 μL was boiled in loading buffer as an input control, and the remaining supernatant was incubated with 100 μl of prewashed anti-Flag G1 affinity resin (number L00432; GenScript, Piscataway, NJ) followed by rotation at 4°C overnight.

Lysis:

Article Title: A gut-derived hormone regulates cholesterol metabolism.
Article Snippet: Article

Article Title: MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria-dependent dephosphorylation of EGFR.
Article Snippet: Cells were lysed in ice-cold lysis buffer (50 mmol/L TrisHCl [pH 7.5], 150mmol/LNaCl, 1% Triton X-100, 1mmol/L EDTA) with 1 × protease inhibitor cocktail (C0001, TargetMol, Boston, MA, USA) for 30 min. Heterologously expressed MFN2-Flag and EGFR-HA were subjected to anti-Flag G1 affinity resin (L00432, GenScript, Nanjing, Jiangsu, China) or anti-HA Magnetic Beads (#88836, Thermo-Fisher Scientific, Waltham, MA, USA).

Article Title: MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria‐dependent dephosphorylation of EGFR
Article Snippet: Cells were lysed in ice‐cold lysis buffer (50 mmol/L Tris‐HCl [pH 7.5], 150 mmol/L NaCl, 1% Triton X‐100, 1 mmol/L EDTA) with 1 × protease inhibitor cocktail (C0001, TargetMol, Boston, MA, USA) for 30 min. Heterologously expressed MFN2‐Flag and EGFR‐HA were subjected to anti‐Flag G1 affinity resin (L00432, GenScript, Nanjing, Jiangsu, China) or anti‐HA Magnetic Beads (#88836, Thermo‐Fisher Scientific, Waltham, MA, USA).

Article Title: The Large Nonstructural Protein (NS1) of Human Bocavirus 1 Directly Interacts with Ku70, Which Plays an Important Role in Virus Replication in Human Airway Epithelia
Article Snippet: The supernatant was then collected, of which 80 μL was boiled in loading buffer as an input control, and the remaining supernatant was incubated with 100 μl of prewashed anti-Flag G1 affinity resin (number L00432; GenScript, Piscataway, NJ) followed by rotation at 4°C overnight.

Protease Inhibitor:

Article Title: A gut-derived hormone regulates cholesterol metabolism.
Article Snippet: Article

Article Title: MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria-dependent dephosphorylation of EGFR.
Article Snippet: Cells were lysed in ice-cold lysis buffer (50 mmol/L TrisHCl [pH 7.5], 150mmol/LNaCl, 1% Triton X-100, 1mmol/L EDTA) with 1 × protease inhibitor cocktail (C0001, TargetMol, Boston, MA, USA) for 30 min. Heterologously expressed MFN2-Flag and EGFR-HA were subjected to anti-Flag G1 affinity resin (L00432, GenScript, Nanjing, Jiangsu, China) or anti-HA Magnetic Beads (#88836, Thermo-Fisher Scientific, Waltham, MA, USA).

Article Title: MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria‐dependent dephosphorylation of EGFR
Article Snippet: Cells were lysed in ice‐cold lysis buffer (50 mmol/L Tris‐HCl [pH 7.5], 150 mmol/L NaCl, 1% Triton X‐100, 1 mmol/L EDTA) with 1 × protease inhibitor cocktail (C0001, TargetMol, Boston, MA, USA) for 30 min. Heterologously expressed MFN2‐Flag and EGFR‐HA were subjected to anti‐Flag G1 affinity resin (L00432, GenScript, Nanjing, Jiangsu, China) or anti‐HA Magnetic Beads (#88836, Thermo‐Fisher Scientific, Waltham, MA, USA).

Article Title: The Large Nonstructural Protein (NS1) of Human Bocavirus 1 Directly Interacts with Ku70, Which Plays an Important Role in Virus Replication in Human Airway Epithelia
Article Snippet: The supernatant was then collected, of which 80 μL was boiled in loading buffer as an input control, and the remaining supernatant was incubated with 100 μl of prewashed anti-Flag G1 affinity resin (number L00432; GenScript, Piscataway, NJ) followed by rotation at 4°C overnight.

Magnetic Beads:

Article Title: A gut-derived hormone regulates cholesterol metabolism.
Article Snippet: Article

Article Title: MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria-dependent dephosphorylation of EGFR.
Article Snippet: Cells were lysed in ice-cold lysis buffer (50 mmol/L TrisHCl [pH 7.5], 150mmol/LNaCl, 1% Triton X-100, 1mmol/L EDTA) with 1 × protease inhibitor cocktail (C0001, TargetMol, Boston, MA, USA) for 30 min. Heterologously expressed MFN2-Flag and EGFR-HA were subjected to anti-Flag G1 affinity resin (L00432, GenScript, Nanjing, Jiangsu, China) or anti-HA Magnetic Beads (#88836, Thermo-Fisher Scientific, Waltham, MA, USA).

Article Title: MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria‐dependent dephosphorylation of EGFR
Article Snippet: Cells were lysed in ice‐cold lysis buffer (50 mmol/L Tris‐HCl [pH 7.5], 150 mmol/L NaCl, 1% Triton X‐100, 1 mmol/L EDTA) with 1 × protease inhibitor cocktail (C0001, TargetMol, Boston, MA, USA) for 30 min. Heterologously expressed MFN2‐Flag and EGFR‐HA were subjected to anti‐Flag G1 affinity resin (L00432, GenScript, Nanjing, Jiangsu, China) or anti‐HA Magnetic Beads (#88836, Thermo‐Fisher Scientific, Waltham, MA, USA).

Article Title: The Large Nonstructural Protein (NS1) of Human Bocavirus 1 Directly Interacts with Ku70, Which Plays an Important Role in Virus Replication in Human Airway Epithelia
Article Snippet: The supernatant was then collected, of which 80 μL was boiled in loading buffer as an input control, and the remaining supernatant was incubated with 100 μl of prewashed anti-Flag G1 affinity resin (number L00432; GenScript, Piscataway, NJ) followed by rotation at 4°C overnight.

Control:

Article Title: A gut-derived hormone regulates cholesterol metabolism.
Article Snippet: Article

Article Title: MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria-dependent dephosphorylation of EGFR.
Article Snippet: Cells were lysed in ice-cold lysis buffer (50 mmol/L TrisHCl [pH 7.5], 150mmol/LNaCl, 1% Triton X-100, 1mmol/L EDTA) with 1 × protease inhibitor cocktail (C0001, TargetMol, Boston, MA, USA) for 30 min. Heterologously expressed MFN2-Flag and EGFR-HA were subjected to anti-Flag G1 affinity resin (L00432, GenScript, Nanjing, Jiangsu, China) or anti-HA Magnetic Beads (#88836, Thermo-Fisher Scientific, Waltham, MA, USA).

Article Title: MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria‐dependent dephosphorylation of EGFR
Article Snippet: Cells were lysed in ice‐cold lysis buffer (50 mmol/L Tris‐HCl [pH 7.5], 150 mmol/L NaCl, 1% Triton X‐100, 1 mmol/L EDTA) with 1 × protease inhibitor cocktail (C0001, TargetMol, Boston, MA, USA) for 30 min. Heterologously expressed MFN2‐Flag and EGFR‐HA were subjected to anti‐Flag G1 affinity resin (L00432, GenScript, Nanjing, Jiangsu, China) or anti‐HA Magnetic Beads (#88836, Thermo‐Fisher Scientific, Waltham, MA, USA).

Article Title: The Large Nonstructural Protein (NS1) of Human Bocavirus 1 Directly Interacts with Ku70, Which Plays an Important Role in Virus Replication in Human Airway Epithelia
Article Snippet: The supernatant was then collected, of which 80 μL was boiled in loading buffer as an input control, and the remaining supernatant was incubated with 100 μl of prewashed anti-Flag G1 affinity resin (number L00432; GenScript, Piscataway, NJ) followed by rotation at 4°C overnight.

Incubation:

Article Title: A gut-derived hormone regulates cholesterol metabolism.
Article Snippet: Article

Article Title: MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria-dependent dephosphorylation of EGFR.
Article Snippet: Cells were lysed in ice-cold lysis buffer (50 mmol/L TrisHCl [pH 7.5], 150mmol/LNaCl, 1% Triton X-100, 1mmol/L EDTA) with 1 × protease inhibitor cocktail (C0001, TargetMol, Boston, MA, USA) for 30 min. Heterologously expressed MFN2-Flag and EGFR-HA were subjected to anti-Flag G1 affinity resin (L00432, GenScript, Nanjing, Jiangsu, China) or anti-HA Magnetic Beads (#88836, Thermo-Fisher Scientific, Waltham, MA, USA).

Article Title: MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria‐dependent dephosphorylation of EGFR
Article Snippet: Cells were lysed in ice‐cold lysis buffer (50 mmol/L Tris‐HCl [pH 7.5], 150 mmol/L NaCl, 1% Triton X‐100, 1 mmol/L EDTA) with 1 × protease inhibitor cocktail (C0001, TargetMol, Boston, MA, USA) for 30 min. Heterologously expressed MFN2‐Flag and EGFR‐HA were subjected to anti‐Flag G1 affinity resin (L00432, GenScript, Nanjing, Jiangsu, China) or anti‐HA Magnetic Beads (#88836, Thermo‐Fisher Scientific, Waltham, MA, USA).

Article Title: The Large Nonstructural Protein (NS1) of Human Bocavirus 1 Directly Interacts with Ku70, Which Plays an Important Role in Virus Replication in Human Airway Epithelia
Article Snippet: The supernatant was then collected, of which 80 μL was boiled in loading buffer as an input control, and the remaining supernatant was incubated with 100 μl of prewashed anti-Flag G1 affinity resin (number L00432; GenScript, Piscataway, NJ) followed by rotation at 4°C overnight.



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